For my PhD thesis project, I adapted the optogenetic tool CLASP (Controllable Light Activated Shuttling and
Plasma membrane sequestration) to mammalian cells. This figure illustrates the mechanism of CLASP.
CLASP utilizes two asLOV2 domains, blue light sensitive proteins derived from Avena Sativa, to sequester protein cargo at the plasma membrane. In response to blue light, the asLOV2 domains release the cargo and expose a nuclear localization signal.